make
TCR
cloning software
Sample Name
Repertoire file
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Species
Human
Mouse
Clone
Most Frequent TCRs
How many?
Match Target TCRs
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Randomly sampled TCRs
How many?
Positive Controls
Use N nucleotide replicates per CDR3
Number of replicates per CDR3
Minimum Hamming distance
α/β Constant Region
Human + disulfide
Human + α/β domain switching
Mouse + disulfide
Human + disulfide-T2A-CD3ζ
Human + disulfide-T2A-puroR
Human + disulfide-T2A-staygold
Mouse + disulfide-T2A-puroR
Human
Mouse
γ/δ Constant Region:
Human
Show Advanced Options
Nested demultiplexing primer set
No two-step nested demultiplexing (max 383 TCRs)
Plate set 1
Plate set 2
Plate set 3
Plate set 4
Plate set 5
Plate set 6
Plate set 7
Plate set 8
Plate set 9
Array TCRs on plate type
384
96
T Cell Subset Selection
A/B cells
Include
Exclude
G/D cells
Include
Exclude
MAIT cells
Include
Exclude
CAIT cells
Include
Exclude
Example Files
Protocols
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makeTCR cloning software
makeTCR cloning software
makeTCR cloning software